AI Reality Check

Laboratory validation

We validate our AI scientist K Pro’s answers in our state-of-the-art wet laboratory.

What is Lab validation?

Lab validation

How do you check AI predictions about biology?

AI analysis is not enough. You have to test predictions in real biological systems.

Owkin uses K Pro to inform our ongoing work in selected research areas. When K Pro makes an interesting prediction about new biology, we test that prediction in our wet lab.

The results tell us if K Pro is providing the level of biological insight we need and the data can be fed back into K Pro to improve its future responses.

More about K Pro

Asset identification

Laboratory validation drives our asset pipeline

Validating our AI scientist’s data-driven reasoning in our wet lab identifies promising novel molecules.

Case Study

OKN4395

K Pro mapped PGE2/PGD2 and DP1 pathway crosstalk in weeks to enable first-in-human clinical trial design of a new molecule.

Lab validation lead to adoption of OKN4395, a first-in-class EP2/EP4/DP1 triple inhibitor, now in Phase 1a clinical trial.

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Case Study

OKN3201

Solid tumors are hard to hit with ADCs. K Pro's ADC tools optimized the design of a bispecific ADC for sharpened tumor targeting.

Lab validation lead to adoption of OKN3201, a potential first-in-class bispecific ADC for solid tumors.

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Technology

State-of-the-art in vitro methodology

Our lab uses patient-derived organoids to more faithfully recapitulate the biological environment in real patients - supported with a variety of immunohistochemical techniques.

In vitro models

78 cell lines
Patient derived organoids, TILs & CAFs 
(Bladder & Ovarian Cancer)
Healthy blood derived PBMCs, T cells, NK cells, monocytes, dendritic cells
Co-culture Tumor/immune cells
3D spheroids
Gene perturbed models
(siRNA, shRNA, lentiviruses)

Functional assays

Cell phenotyping
(FACS)
Biomarker validation at protein level
(ELISA, MSD, Lumit, Western Blot)
Biomarker validation at gene level
(RT-qPCR, sequencing > CRO)
Viability via ATP quantification
(Luminescence)
Viability via live/dead assessment
(FACS)
Semi-automated HTS drug screening
(Integra Assist robot)

Building towards a semi-autonomous lab-in-the-loop

Owkin is developing the connection between our AI scientist and our wet lab. In the future, K Pro will be able to autonomously request wet lab validation for key experiments during its autonomous action.

K Pro generates hypothesis

K Pro uses state-of-the-art frontier reasoning models, multimodal patient data and AI analysis tools to predict new biology.

Lab sets up experimental design

Choose and prepare model system, assay concentrations and timepoints to test prediction.

Lab performs experiment

Test K Pro’s hypothesis through drug assays and genetic perturbation.

Automated results exported to K Pro

Automated microscopy reads experimental results from cells and exports report to K Pro.

K Pro learns from this iteration

Lab data is analysed automatically and used to validate hypothesis for reinforcement learning.

Patient Validation

AI for patients, always

This is how we deliver real world impact to patients at a speed never-before possible.

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What is agentic AI?

How is agentic AI used in Healthcare?

Find out how Owkin is focussed on using agentic AI to understand biology better than existing science.

Find out how